mouse igg2b isotype control (clone mpc-11)  (BioXcel Inc)

Journal: Cell

Article Title: B cells and T follicular helper cells mediate response to checkpoint inhibitors in high mutation burden mouse models of breast cancer.

doi: 10.1016/j.cell.2019.10.028

Figure Lengend Snippet: (A) RNA-seq signatures for sensitive tumors at 7 days (5mm= day 0/ treatment initiation) without or with anti-PD1/anti-CTLA4 therapy. (B) Flow cytometry results for CD8+ cells and CD4+ using memory markers (Cd44, Cd62L). (C) Flow cytometry of tumor infiltrating B cells with or without ICI therapy. On the right shows staining for B cells gated for activation markers. (D) Quantification of flow cytometry for activated B cells (B220+, Cd19+ or Cd20+, MHC II +, Cd80+ or Cd86+). (E) IHC staining for IgG-kappa chain in KPB25Luv tumors. (F) IgG binding assay showing serum-IgG binding (Fitc+) to KPB25Luv cells. (G) Quantification of Fitc+ cells in IgG binding assay for KP25Luv cells and off-target binding. (H) Quantification of Fitc+ IgG binding assay for T11-Apobec cells following reabsorption on off-target cells. In boxplots, bars signify the mean and standard deviation. The p-values are two-tailed from unmatched T-tests. All tumors collected after 7days of treatment or non-treatment.

Article Snippet: Mouse IgG2b Isotype Control (clone MPC-11) , Bioxcel , Cat: BE0086; RRID: AB_1107791.

Techniques: RNA Sequencing, Flow Cytometry, Staining, Activation Assay, Immunohistochemistry, Binding Assay, Standard Deviation, Two Tailed Test

Journal: Cell

Article Title: B cells and T follicular helper cells mediate response to checkpoint inhibitors in high mutation burden mouse models of breast cancer.

doi: 10.1016/j.cell.2019.10.028

Figure Lengend Snippet: (A) Flow cytometry for B cells in KPB25Luv tumors after 7 days of ICI and CD4+ T cell depletion. (B) Quantification of results from A. (C) X-Y plot of IgG and CIBERSORT Tfh T cell signatures in mRNA-seq of sensitive tumors at day 7. (D) Boxplot of CIBERSORT Tfh T cell signature levels in sensitive tumors (mRNA-seq) at day 7. (E) X-Y plot of IgG signature and Il21 mRNA in mRNA-seq of sensitive tumors at day 7. (F) Boxplot of Il21 mRNA levels in sensitive tumors (mRNA-seq) at day 7. (G) Flow cytometry results for activated B cells in T11-Apobec & KPB25Luv tumors during Tfh/IL21 blockade. (H) IHC staining for IgG-kappa chain in KPB25Luv tumors during Tfh/IL21 blockade. (I) Survival for T11-Apobec bearing mice during ICI therapy and Tfh/IL21 blockade. (J) Survival for KPB25Luv bearing mice during ICI therapy and Tfh/IL21 blockade. (K) Western blot for serum IgG in Igmi and Balbc mice with T11-Apobec tumors. The blue bars mark Igmi mouse sera, purple note Balbc sera. (L) 21 day acute response in Igmi and Balbc control mice with T11-Apobec tumors. (M) Survival of Igmi mice withT11-Apobec tumors and treated with anti-PD1/anti-CTLA4 therapy in contrast to Balbc controls. (N) Survival in KPB25Luv tumor bearing mice treated with ICI therapy or ICI therapy with CD16/32 blockade . In Kaplan-Meier plots, p-values are from Log-rank (Mantel-Cox) tests. Boxplots show the mean and standard deviation. The p-values are two-tailed from standard T-tests. In X-Y plots, p-values were determined by linear regression analysis. The asterisks denote significance (***, p<0.0001, *, P<0.05).

Article Snippet: Mouse IgG2b Isotype Control (clone MPC-11) , Bioxcel , Cat: BE0086; RRID: AB_1107791.

Techniques: Flow Cytometry, Immunohistochemistry, Western Blot, Control, Standard Deviation, Two Tailed Test

Journal: Cell

Article Title: B cells and T follicular helper cells mediate response to checkpoint inhibitors in high mutation burden mouse models of breast cancer.

doi: 10.1016/j.cell.2019.10.028

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse IgG2b Isotype Control (clone MPC-11) , Bioxcel , Cat: BE0086; RRID: AB_1107791.

Techniques: Control, Virus, Recombinant, Staining, Multiplex Assay, Enzyme-linked Immunosorbent Assay, Microarray, Plasmid Preparation, Software, Membrane, Transfection

Journal: Cell

Article Title: B cells and T follicular helper cells mediate response to checkpoint inhibitors in high mutation burden mouse models of breast cancer.

doi: 10.1016/j.cell.2019.10.028

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse IgG2b Isotype Control (clone MPC-11) , Bioxcel , Cat: BE0086; RRID: AB_1107791.

Techniques: Control, Virus, Recombinant, Staining, Multiplex Assay, Enzyme-linked Immunosorbent Assay, Microarray, Plasmid Preparation, Software, Membrane, Transfection